Journal: PLOS One

Article Title: A novel peptide mimetic, brilacidin, for combating multidrug-resistant Neisseria gonorrhoeae

doi: 10.1371/journal.pone.0325722

Figure Lengend Snippet: A) Propidium iodide fluorescence after treating N. gonorrhoeae with either azithromycin or brilacidin to predict the permeabilization of the cytoplasmic membrane. B) Percentage of ATP leakage from N. gonorrhoeae treated with brilacidin or azithromycin relative to Triton X-100 (positive control with complete ATP leakage). Asterisks denote statistically significant differences between test agents and DMSO (untreated), * (P < 0.05), ** (P < 0.01), and **** (P < 0.0001) as determined by one-way ANOVA.

Article Snippet: Media and reagents including McCoy’s 5A medium and hematin (Sigma Aldrich, St. Louis, MO, USA), triton X-100 (Acros Organics, Fair Lawn, NJ, USA), BacTiter-Glo reagent (Promega Corporation, Madison, WI, USA), Propidium iodide (PI) and nicotinamide adenine dinucleotide (NAD) (Chem-Impex International, Wood Dale, IL, USA), MTS (3-(4,5-dimethylthia- zol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (Abcam, Waltham, MA, USA), and brucella broth, chocolate II agar plates, IsoVitaleX and bovine hemoglobin (Becton, Dickinson and Company, Cockeysville, MD, USA), were obtained from chemical vendors.

Techniques: Fluorescence, Membrane, Positive Control

Journal: Experimental and Therapeutic Medicine

Article Title: Scutellaria barbata D. Don inhibits growth and induces apoptosis by suppressing IL-6-inducible STAT3 pathway activation in human colorectal cancer cells

doi: 10.3892/etm.2015.2692

Figure Lengend Snippet: Effect of EESB on the HT-29 cell cycle. Cells were pretreated with various concentration of EESB for 1 h, followed by stimulation with 10 ng/ml IL-6 for 24 h. (A) Cells were stained with propidium iodide and analyzed by fluorescence-activated cell sorting. The proportion of DNA in the S phase was calculated using ModFit LT version 3.0 software. (B) Quantification of fluorescence-activated cell sorting analysis. The data shown are averages with standard deviation from 3 independent experiments. #P<0.05 vs. cells treated with IL-6 but not EESB. EESB, ethanol extract of Scutellaria barbata D. Don; IL-6, interleukin-6.

Article Snippet: The HT-29 cell cycle progression was determined through flow cytometric analysis using a propidium iodide (PI) staining cell cycle assay kit (BD Biosciences, Franklin Lakes, NJ, USA).

Techniques: Concentration Assay, Staining, Fluorescence, FACS, Software, Standard Deviation

Journal: Experimental and Therapeutic Medicine

Article Title: Scutellaria barbata D. Don inhibits growth and induces apoptosis by suppressing IL-6-inducible STAT3 pathway activation in human colorectal cancer cells

doi: 10.3892/etm.2015.2692

Figure Lengend Snippet: Effect of EESB on HT-29 cell apoptosis. Cells were pretreated with various concentration of EESB for 1 h, followed by stimulation with 10 ng/ml IL-6 for 24 h. (A) Cells were collected and stained with Annexin V/PI, followed by fluorescence-activated cell sorting analysis. Double-negative stained cells indicate the live cell population; Annexin V-positive/PI-negative stained cells and Annexin V/PI double-positive stained cells represent early and late apoptosis, respectively; Annexin V-negative and PI-positive stained cells show dead cells. (B) Quantification of fluorescence-activated cell sorting analysis. The data shown are averages with standard deviation from 3 independent experiments. #P<0.05 vs. cells treated with IL-6 but not EESB. EESB, ethanol extract of Scutellaria barbata D. Don; IL-6, interleukin-6; UL, upper left; UR, upper right; LR, lower right; LL, lower left; PI, propidium iodide; FITC, fluorescein isothiocyanate.

Article Snippet: The HT-29 cell cycle progression was determined through flow cytometric analysis using a propidium iodide (PI) staining cell cycle assay kit (BD Biosciences, Franklin Lakes, NJ, USA).

Techniques: Concentration Assay, Staining, Fluorescence, FACS, Standard Deviation

Journal: Nutrients

Article Title: Curcumin Anti-Apoptotic Action in a Model of Intestinal Epithelial Inflammatory Damage

doi: 10.3390/nu9060578

Figure Lengend Snippet: Representative flow cytograms of Annexin V-propidium iodide staining, for each experimental condition.

Article Snippet: The apoptosis rate of HT29 was analyzed by flow cytometry using FITC-conjugated Annexin V (An) and propidium iodide staining (Annexin V-FITC Apoptosis Detection Kit, Immunostep, Salamanca, Spain), according to the producer’s instructions.

Techniques: Staining

Journal: Nutrients

Article Title: Curcumin Anti-Apoptotic Action in a Model of Intestinal Epithelial Inflammatory Damage

doi: 10.3390/nu9060578

Figure Lengend Snippet: The effect of curcumin pre-treatment on HT29 apoptosis. The HT29 cell line was pre-incubated with curcumin at different time intervals and then stimulated with IFNγ for an additional 48 h. Programmed cell death is represented by Annexin V-positive cells (An+) expressed as a fold increase compared to untreated cells. Results are expressed as means ± SD of three independent experiments performed in duplicate. Data were analyzed with one-way ANOVA using the Bonferroni post-test (a: p < 0.05; c: p < 0.001).

Article Snippet: The apoptosis rate of HT29 was analyzed by flow cytometry using FITC-conjugated Annexin V (An) and propidium iodide staining (Annexin V-FITC Apoptosis Detection Kit, Immunostep, Salamanca, Spain), according to the producer’s instructions.

Techniques: Incubation